| Institution | Boston University |
| Passage Number | 27 |
| Starting Materials | PBMCs |
| Culture Platform | mTeSR adapted |
| IPSC Reprogramming Method | Sendai Virus |
| File Name | Document Type |
|---|---|
| Plurip stain PiZZ6S-3 MH compressed .pdf | Immunofluorescence Staining |
| PiZZ6S-3 KT.pdf | Karyotype |
| PiZZ6-S3 KT.pdf | Karyotype |
| PiZZ6 STR analysis.pdf | STR Analysis |
| Designation | Alias | Description | Reporter | Reporter Type | Reporter Technique | Knockout | Gene Correction | Other Text |
|---|---|---|---|---|---|---|---|---|
| PiZZ6-MM | PIZZ6S-3-MM2 | bi-allelic CRISPR correction of SERPINA1 Z mutation | No | No | Yes - Homozygous | CRISPR-Cas9 editing with DNA oligo repair template | ||
| PiZZ6-MZ | PiZZ6S-3-MZ13 | mono-allelic CRISPR correction of SERPINA1 Z mutation | No | No | Yes - Heterozygous | CRISPR-Cas9 editing with DNA oligo repair template |
| Variant Gene | Variant Description |
|---|---|
| SERPINA1 | Glu342Lys |
| Link Description | URL |
|---|---|
| Reference | Kotton/Wilson Labs |