| Institution | Boston University |
| Passage Number | 36 |
| Starting Materials | Dermal Fibroblasts |
| Culture Platform | mTeSR adapted |
| IPSC Reprogramming Method | EF1a-hSTEMCCA4 loxp lentiviral infection |
| File Name | Document Type |
|---|---|
| PiZZ100-3-CR1.pdf | Immunofluorescence Staining |
| 100-3-Cr1 P37(11) 9:10:19 matrigel.pdf | Karyotype |
| PiZZ100 STR analysis.pdf | STR Analysis |
| Designation | Alias | Description | Reporter | Reporter Type | Reporter Technique | Knockout | Gene Correction | Other Text |
|---|---|---|---|---|---|---|---|---|
| PiZZ100-MZ | 100-3-Cr1-MZ58 | mono-allelic CRISPR correction of Z mutation | No | No | Yes - Heterozygous | CRISPR-Cas9 editing with DNA oligo repair template | ||
| PiZZ100-MM | 100-3-Cr1-MM56 | bi-allelic CRISPR correction of Z mutation | No | No | Yes - Homozygous | CRISPR-Cas9 editing with DNA oligo repair template |
| Variant Gene | Variant Description |
|---|---|
| SERPINA1 | Glu342Lys |