| Institution | Boston University |
| Passage Number | 21 |
| Starting Materials | PBMCs |
| Culture Platform | mTeSR adapted |
| IPSC Reprogramming Method | EF1a-hSTEMCCA4 loxp lentiviral infection |
| File Name | Document Type |
|---|---|
| BU3 NGST KT.pdf | Karyotype |
| BU3 NGST KT.pdf | Karyotype |
| BU3 NGST STR Analysis.pdf | STR Analysis |
| Description | Parent | Reporter | Reporter Type | Reporter Technique | Knockout | Gene Correction | Other Text |
|---|---|---|---|---|---|---|---|
| NKX2.1-GFP and SFTPC-tdTomato knock-in reporter | BU3 | No | No |
| Associated Gene Expression Data |
|---|
| https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE137805 |
| https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE152654 |
| Designation | Alias | Description | Reporter | Reporter Type | Reporter Technique | Knockout | Gene Correction | Other Text |
|---|---|---|---|---|---|---|---|---|
| BU3 NGST CRISPRi | Yes | GFP | Knockin - NKX2-1 | No | No | dox-inducible dCas9-KRAB construct (Mandegar et al. Cell Stem Cell 18, 541–553 2016) knocked into AAVS1 locus | ||
| BU3 NGST KRAS3 | BU3 NGST-TetOn:KRASG12D | Yes - KRASG12D dox inducible oncogene knocked in to the human AAVS1 locus; also has knockin NKX2-1GFP;SFTPCtdTomato reporters | GFP | Knockin - SFTPC | No | No | published in Dost/Kotton/Kim Cell Stem Cell 2020. PMID: 32891189 |