Designation
BU3 NGST KRAS3
Alias
BU3 NGST-TetOn:KRASG12D
| Species |
Cell Type |
Disease |
Ethnicity |
Race |
Secondary Race |
Karyotype |
Age |
Parent |
Avail |
| Human |
IPSC |
Normal |
None Reported
|
White |
|
46XY
|
33
|
BU3 NGST
|
Yes
|
|
|
|
|
Institution
|
Boston University
|
|
Passage Number
|
27
|
|
Starting Materials
|
PBMCs
|
|
Culture Platform
|
mTeSR adapted
|
|
IPSC Reprogramming Method
|
EF1a-hSTEMCCA4 loxp lentiviral infection
|
|
Description
|
Parent
|
Reporter
|
Reporter Type
|
Reporter Technique
|
Knockout
|
Gene Correction
|
Other Text
|
|
|
BU3 NGST
|
Yes
- KRASG12D dox inducible oncogene knocked in to the human AAVS1 locus; also has knockin NKX2-1GFP;SFTPCtdTomato reporters
|
GFP
|
Knockin - SFTPC
|
No
|
No
|
published in Dost/Kotton/Kim Cell Stem Cell 2020. PMID: 32891189
|