Designation
BU3 NGST CRISPRi
| Species |
Cell Type |
Disease |
Ethnicity |
Race |
Secondary Race |
Karyotype |
Age |
Parent |
Avail |
| Human |
IPSC |
None Reported |
Not Hisp/Lat
|
White |
White-European |
46XY
|
32
|
BU3 NGST
|
Yes
|
|
|
|
|
Institution
|
Boston University
|
|
Passage Number
|
44
|
|
Starting Materials
|
PBMCs
|
|
Culture Platform
|
mTeSR adapted
|
|
IPSC Reprogramming Method
|
EF1a-hSTEMCCA4 loxp lentiviral infection
|
|
Description
|
Parent
|
Reporter
|
Reporter Type
|
Reporter Technique
|
Knockout
|
Gene Correction
|
Other Text
|
|
|
BU3 NGST
|
Yes
|
GFP
|
Knockin - NKX2-1
|
No
|
No
|
dox-inducible dCas9-KRAB construct (Mandegar et al. Cell Stem Cell 18, 541–553 2016) knocked into AAVS1 locus
|
|
Link Description
|
URL
|
|
Reference
|
Werder et al. Sci Adv 2022.
|