iPSC Lines - Detail

Designation
BU3 NGST CRISPRi
Description
Species Cell Type Disease Ethnicity Race Secondary Race Karyotype Age Parent Avail
Human IPSC None Reported Not Hisp/Lat White White-European 46XY 32 BU3 NGST Yes


 
Institution Boston University
Passage Number
Starting Materials PBMCs
Culture Platform mTeSR adapted
IPSC Reprogramming Method EF1a-hSTEMCCA4 loxp lentiviral infection
 
 
 
 
File Name Document Type
BU3 NGST CRISPRi KT.pdf Karyotype
 
 
Description Parent Reporter Reporter Type Reporter Technique Knockout Gene Correction Other Text
BU3 NGST Yes GFP Knockin - NKX2-1 No No dox-inducible dCas9-KRAB construct (Mandegar et al. Cell Stem Cell 18, 541–553 2016) knocked into AAVS1 locus