Institution | Boston University |
Passage Number | 60 |
Starting Materials | PBMCs |
Culture Platform | mTeSR adapted |
IPSC Reprogramming Method | EF1a-hSTEMCCA4 loxp lentiviral infection |
Description | Parent | Reporter | Reporter Type | Reporter Technique | Knockout | Gene Correction | Other Text |
---|---|---|---|---|---|---|---|
Parent BU3 AG line CRISPR/Cas9 mutagenized with homozygous W308R ABCA3 mutation | BU3 AG (wildtype ABCA3:GFP) | Yes - ABCA3:GFP | GFP | Knockin - ABCA3 | No | No | Homozygous CRISPR/Cas9 mutagenesis |