iPSC Lines - Detail

Designation
BU3 AG (wildtype ABCA3:GFP)
Alias
BU3 AG23 Cr6, BU3 ABCA3:GFP
Description
ABCA3-GFP homozygous fusion knock-in reporter for iAEC2
Species Cell Type Disease Ethnicity Race Secondary Race Karyotype Age Parent Avail
Human IPSC Normal None Reported White 46XY 32 BU3 Yes


 
Institution Boston University
Passage Number 41
Starting Materials PBMCs
Culture Platform mTeSR adapted
IPSC Reprogramming Method None Reported
 
 
 
 
File Name Document Type
BU3-AG KT.pdf Karyotype
 
 
Description Parent Reporter Reporter Type Reporter Technique Knockout Gene Correction Other Text
ABCA3-GFP homozygous fusion knock-in reporter for iAEC2 BU3 Yes - ABCA3:GFP GFP Knockin - ABCA3 No No
 
 
 
 
Designation Alias Description Reporter Reporter Type Reporter Technique Knockout Gene Correction Other Text
BU3 AG E690K BU3_AG23_CR6_E690K_13; BU3 AG L101P Parent BU3 AG line CRISPR/Cas9-mutagenized with homozygous E690K ABCA3 mutation Yes - ABCA3:GFP GFP Knockin - ABCA3 No No Homozygous CRISPR/Cas9 mutagenesis
BU3 AG (W308R ABCA3:GFP mutant) BU3_AG23_CR6_W308R_168; BU3 AG W308R Parent BU3 AG line CRISPR/Cas9 mutagenized with homozygous W308R ABCA3 mutation Yes - ABCA3:GFP GFP Knockin - ABCA3 No No Homozygous CRISPR/Cas9 mutagenesis
BU3 AG (L101P ABCA3:GFP mutant) BU3 AG_L101P_9; BU3 AG L101P Parent BU3 AG line CRISPR/Cas9-mutagenized with homozygous L101P ABCA3 mutation Yes - ABCA3:GFP GFP Knockin - ABCA3 No No Homozygous CRISPR/Cas9 mutagenesis