Institution | Boston University |
Passage Number | 41 |
Starting Materials | PBMCs |
Culture Platform | mTeSR adapted |
IPSC Reprogramming Method | None Reported |
Description | Parent | Reporter | Reporter Type | Reporter Technique | Knockout | Gene Correction | Other Text |
---|---|---|---|---|---|---|---|
ABCA3-GFP homozygous fusion knock-in reporter for iAEC2 | BU3 | Yes - ABCA3:GFP | GFP | Knockin - ABCA3 | No | No |
Designation | Alias | Description | Reporter | Reporter Type | Reporter Technique | Knockout | Gene Correction | Other Text |
---|---|---|---|---|---|---|---|---|
BU3 AG E690K | BU3_AG23_CR6_E690K_13; BU3 AG L101P | Parent BU3 AG line CRISPR/Cas9-mutagenized with homozygous E690K ABCA3 mutation | Yes - ABCA3:GFP | GFP | Knockin - ABCA3 | No | No | Homozygous CRISPR/Cas9 mutagenesis |
BU3 AG (W308R ABCA3:GFP mutant) | BU3_AG23_CR6_W308R_168; BU3 AG W308R | Parent BU3 AG line CRISPR/Cas9 mutagenized with homozygous W308R ABCA3 mutation | Yes - ABCA3:GFP | GFP | Knockin - ABCA3 | No | No | Homozygous CRISPR/Cas9 mutagenesis |
BU3 AG (L101P ABCA3:GFP mutant) | BU3 AG_L101P_9; BU3 AG L101P | Parent BU3 AG line CRISPR/Cas9-mutagenized with homozygous L101P ABCA3 mutation | Yes - ABCA3:GFP | GFP | Knockin - ABCA3 | No | No | Homozygous CRISPR/Cas9 mutagenesis |