iPSC Lines - Detail

Designation
BU3 NG
Alias
BU3 NKX2.1-GFP
Description
NKX2.1-GFP knock-in reporter
Species Cell Type Disease Ethnicity Race Secondary Race Karyotype Age Parent Avail
Human IPSC Normal Not Hisp/Lat White 46XY 32 BU3 Yes


 
Institution Boston University
Passage Number 35
Starting Materials PBMCs
Culture Platform mTeSR adapted
IPSC Reprogramming Method EF1a-hSTEMCCA4 loxp lentiviral infection
 
 
 
 
File Name Document Type
BU3 NG KT.pdf Karyotype
BU3 NG STR.pdf STR Analysis
 
 
Description Parent Reporter Reporter Type Reporter Technique Knockout Gene Correction Other Text
NKX2.1-GFP knock-in reporter BU3 Yes GFP Knockin - NKX2-1 No No
 
 
Associated Gene Expression Data
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE83310
 
 
Designation Alias Description Reporter Reporter Type Reporter Technique Knockout Gene Correction Other Text
BU3 NGPT Normal Control hiPSC, BU3, carrying a dual reporter system, NKX2-1-GFP; TP63-tdTomato Yes tdTomato Knockin - TP63 No No
BU3 NGP8T BU3 NGPT clone 4.6 Normal Control hiPSC, BU3, carrying a dual reporter system, NKX2-1-GFP and PAX8-tdTomato Yes - dual reporter NKX2-1GFP;PAX8tdTomato tdTomato Knockin - PAX8 and NKX2-1 No No